Voditelj:
Doc.dr.sc. Dinko Mitrečić, dr.med.
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Djelatnici, doktorandi i suradnici:
Ivan Alić, dr.vet.
Prof.dr.sc. Ervina Bilić, dr.med.
Prof.dr.sc. Srećko Gajović, dr.med.
Dunja Gorup, dr.med.
Sandra Grgić, bacc.lab.med.
dr.sc. Katarina Kapuralin
Nina Kosi, dr.med.
Prof.dr.sc. Roland Pochet
Dora Polšek, dr.med.
dr.sc. Marija Renić, dr.med.
dr.sc. Tamara Stipčević
Laboratorij za matične stanice provodi istraživanja temeljena na regenerativnom potencijalu matičnih stanica. Idući u korak s brzim razvojem ovog novonastalog biomedicinskog područja, Laboratorij je uspostavio postupke izolacije stanica iz različitih izvora: u radu se koriste živčane matične stanice podrijetla telencefalona mišjeg zametka, mezenhimske matične stanice podrijetla koštane srži miša, te matične stanica iz subventrikularne zone i hipokampusa odraslih miševa. Istovremeno se razvijaju protokoli uzgoja ljudskih stanica podrijetla pulpe zuba i njušne sluznice te ljudskih induciranih pluripotentnih matičnih stanica. Osnovni smjerovi istraživanja obuhvaćaju in vitro i in vivo analize. In vitro analizama se proučava diferencijacijski i regenerativni potencijal stanica u ovisnosti od egzogenih čimbenika (npr. in vitro model ishemije, ko-kultivacije), opažena se svojstva modificiraju transfekcijom - genetskim manipulacijama, te uzgojem stanica uz potporu biomaterijala (npr. alginat, biopolimeri). Jedan od osnovnih smjerova in vivo istraživanja je analiza regenerativnog potencijala matičnih stanica transplantiranih u mozak miša zahvaćen ishemijskim moždanim udarom. Koristeći brojne egzogene (razni stanični označivači, nanočestice) i endogene biljege (genetski modificirane stanice označene flourescentnim bojama) Laboratorij se specijalizirao za praćenje sudbine stanica nakon transplantacije.
Head:
Assistant Professor Dinko Mitrečić, MD, PhD
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Members, PhD students and main collaborators:
Ivan Alić, DVM
Prof. Ervina Bilić, MD, PhD
Prof. Srećko Gajović, MD, PhD
Dunja Gorup, MD
Sandra Grgić, Bacc.lab.med.
Katarina Kapuralin, PhD
Nina Kosi, MD
Prof. Roland Pochet, PhD
Dora Polšek, MD
Marija Renić, MD, PhD
Tamara Stipčević, PhD
Laboratory for Stem Cells is involved in research activities based on a regenerative potential of stem cells. Keeping the pace with a fast development of this new biomedical field, the Laboratory has established protocols for isolation of cells from various sources: we use neural stem cells from the telencephalon of mouse embryos, mesenchymal stem cells from the mouse bone marrow and stem cells from the adult mouse subventricular zone and hippocampus. Also, we are developing protocols for isolation of human stem cells from the tooth pulp and olfactory mucosa and human induced pluripotent stem cells. The basic directions of research include in vitro and in vivo analyses. In vitro analyses are used to get insight into differentiation and regenerative potential of stem cells in relation to exogenous factors (e.g. in vitro model of ischemia, co-cultivations) and observed features are improved by transfection-genetic manipulations and cultivation of cells using biomaterials (e.g. alginat, biopolymers). One of the major directions of in vivo research is analyses of regenerative potential of stem cells transplanted into the brain of mouse affected by ischemic stroke. Using various exogenous (cell markers, nanoparticles) or endogenous markers (genetically modified cells and fluorescent markers) the Laboratory has been specialized in cell tracing and analyses of cell fates after transplantation.
Using specific cell lines we can recognize differentiation stages (e.g. Nestin-GFP for multipotent stem cells or Thy1-YFP for neurons) or we can recognize some important events, like axonogenesis (Gap43-GFP). Using highly specific equipment for cell transfection (Magnefect Nano II, Nanotherics) and cell tracing equipment (bioluminescence IVIS Spectrum, Perkin Elmer; magnet resonance BIOSPEC 70/20 7T, Bruker) in combination with a whole battery of established morphological and behavioral analyses (morphology, histology, animal behavioral tests) the Laboratory is fully equipped for cell biology research and analyses of the impact of stem cell transplantation on experimental animals. Based on international collaborations with centers which are performing clinical trials, the Laboratory is involved in development and improvement of technology of stem cells for therapeutic applications in ischemic and neurodegenerative diseases. In the period 2009 – 2015 members of the Laboratory have been coordinators of several projects (EU FP7 Regpot, HRZZ, Adris, Bilateral projects with Sweden, Austria and Germany) and in that period 12 CC publications based on stem cells have been published.
- Kosi, N; Alić, I; Kolačević, M; Vrsaljko, N; Jovanov Milošević, N; Sobol, M; Philimonenko, A; Hozák, P; Gajović, S; Pochet, R; Mitrečić, D. Nop2 is expressed during proliferation of neural stem cells and in adult mouse and human brain. Brain Res 2014;1597:65-76.
- Konig, N; Trolle, C; Kapuralin, K; Adamejko, I; Mitrečić, D; Aldskogius, H; Shortland, PJ; Kozlova, E. Murine neural crest stem cells and embryonic stem cell derived neuron precursors survive and differentiate after transplantation in a model of dorsal root avulsion J Tissue Eng Regen Med. 2014 Apr 21.
- Winters, L; Winters, T; Gorup, D; Mitrečić, D; Križ, J; Gajović, S. Expression analysis of genes involved in TLR2-related signaling pathway, inflammation and apoptosis after ischemic brain injury. Neuroscience. 2013 Feb 10;238:87-96.
- Nicaise, C; Mitrečić, D; Pochet, R. Brain and spinal cord affected by amyotrophic lateral sclerosis induce differential growth factors expression in rat mesenchymal and neural stem cells. Neropath Appl Neuro: 2011 Feb;37(2):179-88.
- Mitrečić, D; Nicaise, C; Gajovic, S; Pochet, R. Distribution, differentiation and survival of intravenously administered neural stem cells in a rat model of amyotrophic lateral sclerosis. Cell Transplantation 2010;19(5):537-48.
