Voditelj:
Prof.dr.sc. Nataša Jovanov-Milošević,
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Osoblje:
Maja Horvat, ing.lab.diag.,
Božica Popović, lab.teh.
Laboratorij za immunohistokemiju i in situ hibridizaciju pruža histološka bojanja rezova tkiva različitih dijelova živčanog sustava čovjeka i animalnih modela (miš, štakor, mačka). Kombinirajući različite histološke tehnike prikazuje se ekspresija gena u citoarhitektonski očuvanim uzorcima tkiva. Fiksacija i uklapanje tkiva, vibratomski, parafinski i smrznuti rezovi, rezovi fiksirani na staklo ili “free-floating”, jednostruka, višestruka bojanja i preko 20 različitih histoloških i histokemijskih bojanja specifičnih za neuroznanosti.
Tehnička podrška laboratorija ima preko 20 godina iskustva u obradi moždanog tkiva fetusa i odrasla čovjeka i animalnih modela.
Znanstveni interes:
praćenje prostorne i vremenske distribucije specifičnih proteina u kontekstu razvitka čeonog i limbičkog režnja telencefalona čovjeka, njegove plastičnosti i degeneracije. Poseban interes su morfološko-kemijske značajke prolaznih razvojnih struktura i zona te kortikalnih veza, tijekom normalnog razvitka, nakon hipoksično-ishemičnog oštećenja mozga i u razvojnim anomalijama.
Znanstveni projekti u tijeku:
- 2013. - „Komparativni histološko –MRI istraživački pristup poboljšanju dijagnostike perinatalnih oštećenja mozga čovjeka“ (akronim HIMRICO od Histological-MRI COmparative research) (PI: N. Jovanov Milosevic)
- 2013. – Suradnik istraživač, „Histological, MRI and gene expression analysis of the reorganizational processes in the medial (limbic) wall of developing human cerebrum (PI: M. Vukšić)
- 2011.- 2014. COST BM1001: Brain Extracellular matrix in health an disease” (MC N. Jovanov Milosevic, PI A. Dityatev)
- 2007.- UKF projekt: Neuroimiging, neurogenomics and pharmacogenomics of the frontal lobe connectivity: normal development and abnormalities in developmental disorders, (PI I. Kostović)
- 2006.-“Razvitak i plastičnost kortikalnih putova mozga čovjeka”, dio programa: Razvojna neurobiološka osnova kognitivnih, duševnih i neuroloških bolesti” (PI I. Kostović)
Head:
Associated professor Nataša Jovanov Milošević
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Personnel
Božica Popović, lab. technician,
Maja Horvat, Bachelor of medical laboratory diagnostic
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Laboratory for Immunohistochemistry and In situ hybridization provides histological, immunohistochemical and in situ hybridization (non-radioactive) staining of human and animal nervous system tissue sections. The tissue fixation and embedding, resin, paraffin and frozen sectioning on-slides and free-floating, mono and double immuno-labelling, in situ (non-radioactive) hybridization, and more than 20 different histological and histochemical staining methods specific for neuroscience.
Core staff of the Laboratory has more than two decades of experience in processing fetal and adult human and animal brain tissue.
Scientific scope
Follow up study of spatial-temporal distribution of specific proteins in a context of frontal and limbic lobe development, plasticity and degeneration. Special emphasis is given to morphological-chemical phenotype characterization of the transient developmental features (zones, compartments and cells) and cortical connections of human fetal brain during normal development and in different developmental disorders.
Ongoing research projects
- 2013. –“Histological-MRI comparative research approach for improvement of diagnostic procedures for developmental disorders of the human brain, acronim HIMRICO, (PI: N. Jovanov Milosevic)
- 2013. – “Histological, MRI and gene expression analysis of the reorganizational processes in the medial (limbic) wall of developing human cerebrum (PI: Mario Vukšić)
- 2011.- COST BM1001: Brain Extracellular matrix in health an disease” (PI A. Dityatev, MC, Jovanov Milosevic)
- 2007-2011,- “Neuroimaging, neurogenomics and pharmacogenomics of the frontal lobe connectivity”, Unity through Knowledge Fund, (PI: I. Kostovic)
- Milosevic, Natasa Jovanov; Judas, Milos; Aronica, Eleonora; et al. Neural ECM in laminar organization and connectivity development in healthy and diseased human brain. BRAIN EXTRACELLULAR MATRIX IN HEALTH AND DISEASE, Progress in Brain Research, Volume: 214, Pages: 159-178, 2014
- Kostovic, Ivica; Kostovic-Srzentic, Mirna; Benjak, Vesna; et al. Developmental dynamics of radial vulnerability in the cerebral compartments in preterm infants and neonates. Frontiers in neurology, Volume: 5, Pages: 139, 2014
- Hladnik, Ana; Dzaja, Domagoj; Darmopil, Sanja; Jovanov Milosevic, Natasa; Zdravko Petanjek. Spatio-temporal extension in site of origin for cortical calretinin neurons in primates FRONTIERS IN NEUROANATOMY, Volume: 8, Article Number: 50 Published: JUN 26 2014
- Mlinac K, Jovanov Milošević N, Heffer M, Smalla KH, Schnaar RL, Kalanj Bognar S. Neuroplastin expression in the hippocampus of mice lacking complex gangliosides. J Mol Neurosci.;48(1):161-6, 2012.
- Jovanov-Milošević N, Petrović D, Sedmak G, Vukšić M, Hof PR, Simić G. Human fetal tau protein isoform: Possibilities for Alzheimer's disease treatment Int J Biochem Cell Biol. 44/8: 1290-4, 2012.
- Jovanov-Milošević N, Petanjek Z, Petrović D, Judaš M, Kostović I. Morphology, molecular phenotypes and distribution of neurons in developing human corpus callosum. Eur J Neurosci. 32(9):1423-32, 2010.
- Judaš M, Šimić G, Petanjek Z, Jovanov-Milošević N, Pletikos M, Vasung L, Vikšić M, Kostović I. The Zagreb Collection of human brains: A unique versatile but underexploited resource for the neuroscience community. Ann N Y Acad Sci. 1225 Suppl 1:E105-30. doi: 10.1111/j.1749-6632.2011.05993.x, 2011.
- Vasung L, Jovanov-Milošević N, Pletikos M, Mori S, Judaš M, Kostović I. Prominent periventricular fiber system related to ganglionic eminence and striatum in the human fetal cerebrum. Brain Struct Funct. 215(3-4):237-53, 2011.
- Jovanov-Milošević N, Petanjek Z, Petrović D, Judaš M, Kostović I. Morphology, molecular phenotypes and distribution of neurons in developing human corpus callosum. Eur J Neurosci. 32(9):1423-32, 2010.
- Judaš M, Sedmak G, Pletikos M, Jovanov-Milošević N. Populations of subplate and interstitial neurons in fetal and adult human telencephalon. J Anat. 217(4):381-99., 2010.
- Vasung L, Huang H, Jovanov-Milošević N, Pletikos M, Mori S, Kostović I. Development of axonal pathways in the human fetal fronto-limbic brain: histochemical characterization and diffusion tensor imaging. J Anat. 217(4):400-17, 2010.
- Jovanov-Milošević N, Čuljat M, Kostović I. (2009) Growth of the human corpus callosum: modular and laminar morphogenetic zones. Front Neuroanat. 3(6):1-10, 2009.
- Šimić G, Stanić G, Mladinov M, Jovanov-Milošević N, Kostović I, Hof PR.(2009) Annotation - Does Alzheimer's disease begin in the brainstem? Neuropathol Appl Neurobiol. 35: 432-554, 2009.
